Fig. 3: Retinal protection in dark-reared rd10 mice is associated with decreased lipid peroxidation.

A Study design. Note, graphs B and E-K represent data for mice after one month on treatments; graphs L–Q represent data for mice after three months on treatments. B Group-averaged scotopic ERG waveforms in a representative cohort. C, D Scotopic ERG a-wave C and b-wave D amplitudes at different stages of disease progression in the same mice (repeated measures design). E, F Representative OCT images. G ONL-thickness assessment. H Whole retinal 4-HNE contents, as measured by ELISA (WT, n = 8; rd10-vehicle, n = 9; rd10-TMB, n = 10). I–J Quantification of expression of catalase (CAT) and GFAP from immunoblots (WT, n = 5; rd10-vehicle, n = 12; rd10-TMB, n = 9). K Representative immunoblots from cohort 1. A total of two cohorts (individual experiments) were performed and the data pooled for the analyzes shown in panels (I, J). L, M Representative retinal flat mounts stained with antibodies against M-cone opsin or S(UV)-cone opsin. N, O M-cone and S(UV)-cone counts from retinal flat mounts. P, Q M-cone- and S(UV)-cone-dominant photopic ERG amplitudes. The three-month-long experiments were performed in female rd10 mice. Welch´s t-test (two-tailed) was used to analyze data in G, P and Q. ERG data in (C, D) (age as within-subjects and treatment as between-subjects factor) and cone count data in (N, O) (retinal ___location as within-subjects and treatment as between-subjects factor) were analyzed by two-way RM ANOVA with the Geisser-Greenhouse correction. The statistical analysis performed for H was one-way ANOVA and Bonferroni´s post hoc test, whereas I, J were analyzed by Welch´s ANOVA followed by Dunnett´s T3 tests. The pound signs indicate an ANOVA-significant main effect between treatments: ^#P < 0.05, ^##P < 0.01^. The asterisks mark significant post hoc test effects: *P < 0.05, **P < 0.01, ***P < 0.001, **** P < 0.0001. Data are presented as mean ± SD. Source data are provided as a Source Data file.