Fig. 6: Dietary TMB administration slows retinal degeneration in female Rho^P23H/WT mice.

A Study design. B–C Representative OCT images at 3-months of age. D Representative group-averaged (thick lines) scotopic ERG waveforms (log cd·s/m² = 1.7). Thin traces represent individual mouse responses. E–F Scotopic ERG a-wave E and b-wave F amplitudes. G Longitudinal ONL thickness follow-up throughout the study in one treatment cohort (repeated measures study design). Data analyzed from OCT images. H Representative group-averaged (thick lines) photopic ERG waveforms at 6-months of age. Stimulus was elicited with a green LED that stimulates primarily the M cones (log cd·s/m² = 2.5). I M-cone- J and S(UV)-cone-dominant photopic ERG b-wave-amplitudes. A repeated cross-sectional study. K, L Optic nerve head (ONH)-centered (* shows ONH) central retina images showing M-cone K and S(UV)-cone L populations in a vehicle-treated retina. Dashed-line yellow rectangles indicate 0.75 mm sampling site ___location. M, N ONH-centered central retina images showing M-cone M and S(UV)-cone N populations in a TMB-treated retina. O–P Representative M-cone O and P UV/S-cone images in superior and inferior retina, respectively, at different distances from the ONH. Q–R Inferior retina S(UV)-cone count analysis Q and superior retina M-cone count analysis R. Counting windows sizes were: width 360 µm, height 270 µm. S, T Representative OCT images. Statistical analysis performed for data in graphs E, and F was by Welch´s t-test (two-tailed). Data in graphs G, Q and R were analyzed by two-way RM ANOVA with the Geisser-Greenhouse correction. Data in graphs I and J were analyzed by ordinary two-way ANOVA. ANOVAs were followed by Bonferroni posthoc tests. T-test and Bonferroni post hoc results: *P < 0.05, **P < 0.01. ANOVA between-subjects main effects: ^##P < 0.01, ^####P < 0.0001. Data are presented as mean ± SD. Source data are provided as a Source Data file.