Fig. 2: miR-1204 aggravates angiotensin II (AngII) induced aortic aneurysm and dissection (AAD) formation.

A Scheme of AngII-induced AAD experiments. MiR-1204 agomir or miR-control (miR-ctl) (10 nmol) was administered to 4-month-old male C57BL/6 mice by tail vein injection every 3 d for a total of five times before 4-week AngII-infusion. B AAD incidence. AA aortic aneurysm, AD aortic dissection. Statistical analysis was performed using two-tailed Fisher’s exact test. C Kaplan–Meier curves of survival in the indicated groups. Statistical analysis was performed using log-rank test. D Representative photographs of aortas in the indicated groups. Bar indicates 4 mm. E Representative B-mode ultrasound and doppler ultrasound (DUS) detection of abdominal aortas. The white arrow indicates an aneurysm. F–I Quantification of ascending aorta, aortic arch, descending aorta and abdominal aorta diameters using ultrasound. n = 17 biological replicates for miR-1204 + AngII group. n = 19 biological replicates for other groups. Statistical analysis was performed using a one-way ANOVA followed by Tukey’s multiple comparison test. J Representative images of hematoxylin & eosin (H&E) staining of the abdominal aortas. The black arrow indicates an intimal tear. Upper bar indicates 400 μm. Lower bar indicates 100 μm. Representative images of elastic van Gieson (EVG) staining (left) and elastin degradation grade (right) in the abdominal aorta. n = 17 biological replicates for miR-1204 + AngII group. n = 19 biological replicates for other groups. Statistical analysis was performed using the Kruskal–Wallis test with Dunn’s multiple comparisons test. Scale bar, 100 μm. Representative images of senescence-associated-β-galactosidase (SA-β-gal) staining (left) and quantification of SA-β-gal activity area (right) in the abdominal aorta. n = 17 biological replicates for miR-1204 + AngII group. n = 19 biological replicates for other groups. Statistical analysis was performed using two-tailed Welch’s ANOVA followed by Dunn’s multiple comparison test. Scale bar indicates 50 μm. Data are presented as mean ± SD. Source data are provided as a source data file.