Fig. 1: Cocaine conditioning-induced NAc Npas4+ ensemble is required for CPP. | Nature Communications

Fig. 1: Cocaine conditioning-induced NAc Npas4+ ensemble is required for CPP.

From: NPAS4 supports cocaine-conditioned cues in rodents by controlling the cell type-specific activation balance in the nucleus accumbens

Fig. 1

A Experimental design for Gi-DREADD-mediated manipulation of the NAc Npas4+ ensemble using newly generated NPAS4-TRAP mice. B Experimental timeline for cocaine CPP, including the timing of 4OHT injections for both cocaine conditioning and saline conditioning TRAP experiments. C Inhibition of the cocaine-conditioned NPAS4-TRAP population during the post-test significantly decreases CPP expression (n = 8 mice/group; unpaired t-test, t = 4.688, df = 14, p = 0.0003). D CNO administration in animals injected with a control virus lacking hM4Di has no effect on cocaine CPP expression (n = 6 and 7 mice/group). E Inhibition of the NPAS4 ensemble captured during saline conditioning sessions also has no effect on cocaine CPP expression (n = 8 and 10 mice/group). CPP scores are calculated as time in the cocaine paired chamber minus time in the unpaired chamber during the post-test. Pre-test scores are shown in Fig. S1. Data are shown as mean ± SEM; ***p < 0.001. Source data are provided in the Source Data file.

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