Fig. 4: NPAS4 regulates the preponderant activation of NAc D1-MSNs following cocaine conditioning. | Nature Communications

Fig. 4: NPAS4 regulates the preponderant activation of NAc D1-MSNs following cocaine conditioning.

From: NPAS4 supports cocaine-conditioned cues in rodents by controlling the cell type-specific activation balance in the nucleus accumbens

Fig. 4

A Timeline for cocaine CPP and FOS IHC. B Representative FOS IHC images showing Cre-dependent Npas4 shRNA in the NAc of D2-Cre mice. C Quantification of FOS expression in D1- (n = 6 mice/group; two-way ANOVA, sig. main effect of cell type, f = 105.7, df = 1,20, p = <0.0001) and (D) D2-Cre mice (n = 8 mice/group) after cocaine CPP, including effects on “putative” MSNs after cell type-specific NPAS4 knockdown (two-way ANOVA with multiple comparisons, df = 28, p = 0.0002). E Representative image showing FOS expression in the dlVP surrounding mCherry-labeled fibers from the NAc. F Quantification of FOS expression in the dlVP after NPAS4 knockdown in NAc D2-MSNs (n = 8 and 7 mice/group; unpaired t-test, t = 2.973, df = 12, p = 0.0116). Data shown are mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Source data are provided in the Source Data file.

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