Fig. 3: TBR is required for root growth tolerance to high Zn levels. | Nature Communications

Fig. 3: TBR is required for root growth tolerance to high Zn levels.

From: Natural variation of TBR confers plant zinc toxicity tolerance through root cell wall pectin methylesterification

Fig. 3

a Pictures of seedlings of Col-0, tbr mutants, and the at5g06710 mutant grown on control and high Zn (300 µM) medium at 7 DAG. Scale bars: 5 mm. b Boxplots for root length depicted in (a). The value on the top of each box in high Zn is the relative root length (root length in high Zn / root length in control). The number below each box indicates the number of replicates. Statistical analysis was performed using one-way ANOVA analysis with Tukey’s HSD test (p < 0.05). c Confocal images of the root elongation zone of wildtype, tbr-3 and TBR_Col-0-9 complemented line at 4 DAG. The arrows indicate the border of the maturation zone and meristem zone. Scale bars: 100 µm. d Boxplots for root elongation zone length are depicted in (c). The number below each box indicates the number of replicates. Two times were repeated independently with similar results. Statistical analysis was performed using one-way ANOVA analysis with Tukey’s HSD test (p < 0.05). e Boxplots for root length of TBR_Col-0 T3 complemented lines at 4 DAG. The value on the top of each box in high Zn is the relative root length (root length in high Zn / root length in control). The number below each box indicates the number of replicates. Statistical analysis was performed using one-way ANOVA analysis with Tukey’s HSD test (p < 0.05). For box plots, the horizontal line represents the median value, the lower and upper quartiles represent the 25th and 75th percentile, and the whiskers show the maximum and minimum values. Source data are provided as a Source Data file.

Back to article page