Fig. 2: Biochemical characterization of MicA.
a Schematic overview of microeunicellol-like biosynthetic gene cluster discovered in this study. Supplementary Fig. 3 depicts the microeunicellol-like structure seen in the patent. b Functional characterization of MicA utilizing previously constructed two-step kinase-based GGPP overproduction system, MKI4 (Supplementary Fig. 4)22. Extracted metabolites were analyzed through HPLC by monitoring at 210 nm. “EV” shown in all Figures is referred to “empty vector”. GC-MS spectra of purified 1 (c) and 2 (d). e Chemical structures of 1 and 2. Critical 2D NMR information, including 1H-1H COSY, HMBC, and NOESY correlations, are also shown. f Synthesis of epoxidized derivatives of 1 for X-ray diffraction analysis. Epoxides 1a–1d were synthesized by epoxidation with meta-chloroperoxybenzoic acid (mCPBA). X-ray diffraction analysis of obtained epoxides using Cu Kα (λ = 1.5417 Å) assigned the absolute configuration of 1.
