Fig. 2: Neuronal activation induces rapid reduction of kdm4a expression.
From: Kdm4a is an activity downregulated barrier to generate engrams for memory separation
a Schematic diagram of cultured neurons stimulated by KCl depolarization. KCl 0 h n = 3, KCl 1 h n = 3. Two-tailed unpaired t-test, Fos: t4 = 17.84, **** p < 0.0001; Kdm4a: t4 = 4.532, * p = 0.0106. Data are presented as mean ± s.e.m. b Schematic diagram of in vivo activation of DG using kainic acid (KA). KA 0 h n = 3, KA 1 h n = 3. Two-tailed unpaired t-test, Fos: t4 = 8.200, ** p = 0.0012; Egr1: t4 = 7.078, ** p = 0.0021; Kdm4a: t4 = 4.980, * * p = 0.0076. Data are presented as mean ± s.e.m. c The distribution of Kdm4a signal in the nucleus of NeuN+ cultured neurons (DIV14) treated with KCl 0 h or 1 h. Scale bar, 0.5 μm. d (Left) The Kdm4a intensity in the nucleus of NeuN+ neurons significantly decreased after KCl depolarization. KCl 0 h n = 15; KCl 1 h n = 26. Two-tailed unpaired t-test, t39 = 4.102, *** p = 0.0002. (Right) The number of Kdm4a puncta decreased significantly in KCl depolarized neurons. KCl 0 h n = 15; KCl 1 h n = 22. Two-tailed unpaired t-test, t35 = 5.210, **** p < 0.0001. Box plot indicates the mean, interquartile range and the minimum and maximum. Arbitrary unit, AU. e The intensity of Kdm4a puncta decreased significantly after KCl depolarization. KCl 0 h n = 3, puncta = 4247; KCl 1 h n = 3, puncta = 2946. Two-tailed unpaired t-test, t7191 = 24.08, ****p < 0.0001. Violin plot indicates the mean, interquartile range and the minimum and maximum. Right: Relative frequency distribution of Kdm4a puncta intensity. f Schematic diagram of harvest of fear memory-related DG GCs in vivo. EGFP- n = 3, EGFP+ n = 3. Two-tailed unpaired t-test, Egr1: t4 = 8.902, *** p = 0.0009; EGFP: t4 = 18.84, **** p < 0.0001; Kdm4a: t4 = 3.567, * p = 0.0234. Data are presented as mean ± s.e.m. g Schematic illustrating neuronal activation induced rapid downregulation of Kdm4a expression.
