Fig. 1: Loss of Arid1a in myeloid lineage leads to excessive bone mass.
From: ARID1A safeguards the canalization of the cell fate decision during osteoclastogenesis
a Immunofluorescence staining of ARID1A and CTSK in the distal femur of 4-week-old LysM-Cre;tdT mouse. Arrows indicate LysM+ myeloid lineage ARID1A + CTSK+ osteoclasts (OCs). Scale bar, 100 μm. b Schema for osteoclastic differentiation stages of BMCs in vitro. Bone marrow cells, BMCs. c Immunofluorescence staining of ARID1A and TRAP staining in BMCs after 3 days of RANKL-induction. Yellow boxes are shown at higher magnification on the right. The dashed line outlines the cell boundaries of OCs. Scale bar, 100 μm. d Arid1a mRNA expression in BMCs at indicated days after RANKL-induction, as measured by qPCR. n = 3 biologically independent samples. e ARID1A and MMP9 protein expression in BMCs at indicated days after RANKL-induction, as measured by western blot. f Representative micro-CT image of the distal trabecular bone and cortical bone of femurs from 3-month-old male LysM-Cre;Arid1afl/fl mice and littermate control mice. Thicker arrows indicate the increased bone mass. Scale bar, 2 mm. g Quantification analysis of bone volume/tissue volume ratio (BV/TV), porosity percent (Po), bone mineral density (BMD), trabecular number (Tb.N), trabecular separation (Tb.Sp) and trabecular thickness (Tb.Th) of the distal femoral trabecular bone, mean total crossectional bone area (B. Ar) and mean total crossectional bone perimeter (B. Pm) of cortical bone in the femoral midshaft from 3-month-old male LysM-Cre;Arid1afl/fl mice and littermate control male mice. n = 7. h H&E staining of femurs from 3-week-old male LysM-Cre;Arid1afl/fl mice and littermate control mice. Thicker arrows indicate the increased bone mass. Scale bar, 200 μm. i Von Kossa staining of mesial tibias from 6-week-old male LysM-Cre;Arid1afl/fl mice and littermate control mice. The box in the left panel is shown at a higher magnification on the right. Thicker arrows indicate the increased bone mass. Scale bar, 200 μm. All data in this figure are represented as mean ± SD. One-way analysis of variance (ANOVA) with Tukey’s multiple comparisons test for d. Two-tailed Student’s t-test for g. All experiments were performed in triplicates unless otherwise stated. Source data and exact p values are provided in the Source data file.
