Fig. 8: Brd9 heterozygous knockout partially rescues OC differentiation defects after loss of Arid1a in vivo.

a Representative micro-CT image and b quantification analysis of bone volume/tissue volume ratio (BV/TV), bone mineral density (BMD), trabecular separation (Tb.Sp), and trabecular number (Tb.N) of femurs from 3-month-old female LysM-Cre;Arid1a^fl/fl mice, LysM-Cre;Arid1a^fl/fl;BRD9^fl/+ mice, and littermate control mice. Distal trabecular bone are enlarged on the lower panel. Scale bar, 2 mm. n = 5. c H&E staining and d quantification analysis of trabecular thickness (Tb.Th), Tb.N, and Tb.Sp of the distal femoral trabecular bone from 3-month-old female LysM-Cre;Arid1a^fl/fl mice, LysM-Cre;Arid1a^fl/fl;BRD9^fl/+ mice, and littermate control mice. Scale bar, 200 μm. n = 6. e CTSK immunofluorescence (red) staining and f quantification analysis of the distal femoral trabecular bone from 3-month-old female LysM-Cre;Arid1a^fl/fl mice, LysM-Cre;Arid1a^fl/fl;BRD9^fl/+ mice, and littermate control mice. n = 5. Scale bar, 200 μm. Yellow arrows indicate a positive signal. g The protein expression of ARID1A, STAT1, and BRD9 in BMCs from 4-week-old male LysM-Cre;Arid1a^fl/fl mice, LysM-Cre;Arid1a^fl/fl;BRD9^fl/+ mice, and littermate control mice after RANKL-induction, as measured by western blot. h PAGA graph of cell clusters showing trajectory colored by the timeline and i the expression patterns of Nfatc1 and Stat1 during the trajectory of osteoclastogenesis. j bean plot of the different gene densities of Nfatc1 and Stat1 in cluster 4 between the control group and LysM-Cre;Arid1a^fl/f group. All data in this figure are represented as mean ± SD. One-way ANOVA with Tukey’s multiple comparisons test for b, d, and f. All experiments were performed in triplicates unless otherwise stated. Source data and exact p values are provided in the Source data file.