Fig. 9: The antagonistic function of ARID1A and BRD9 both depend on BRD4 during osteoclastogenesis.
From: ARID1A safeguards the canalization of the cell fate decision during osteoclastogenesis
a The protein expression of STAT1 and CTSK and b STAT1 immunofluorescence (green) in JQ1 or control vector treated BMCs from 4-week-old male LysM-Cre;Arid1afl/fl mice and littermate control mice after RANKL-induction. c GSEA analysis of DEG profiles in bulk RNA-seq between JQ1 or control vector treated BMCs after RANKL-induction. d Co-IP assay with BRD9 antibody (or IgG) in BMCs during osteoclastic induction, followed by immunoblotting of BRD4 and BRD9. e The protein expression of ARID1A and STAT1 in JQ1 or control vector treated BMCs from 4-week-old male LysM-Cre;Arid1afl/fl mice and littermate control mice after RANKL-induction. f The protein expression of ARID1A, MMP9 and NFATc1 in JQ1, iBRD9 or control vector treated BMCs from 4-week-old male LysM-Cre;Arid1afl/fl mice and littermate control mice after RANKL-induction. All experiments were performed in triplicates unless otherwise stated. Source data are provided in the Source data file.
