Fig. 1: An overview of GlycReSoft.

GlycReSoft’s workflow stages. a Search space construction, b LC-MS/MS deisotoping and charge deconvolution, c Scanning the complement glycan ion index for each mass shift, d Full spectrum match re-scoring, e FDR estimation, f MS1 feature extraction and MS2 identification mapping, g Parsimonious updates to glycopeptide-mass shift-MS1 feature assignment, h Relative retention time model, i Glycopeptide revision based upon retention time, j GPSM feature scoring from fragmentation model if available, k Glycosite glycan network smoothing score adjustment if available.