Fig. 1: RNF213 deficiency in CD4+ T cells promoted EAE development and inhibiting Treg cell differentiation. | Nature Communications

Fig. 1: RNF213 deficiency in CD4+ T cells promoted EAE development and inhibiting Treg cell differentiation.

From: RNF213 promotes Treg cell differentiation by facilitating K63-linked ubiquitination and nuclear translocation of FOXO1

Fig. 1

Rnf213fl/fl and Rnf213fl/flCD4Cre mice were immunized with MOG(35-55) peptide in CFA adjuvant and pertussis toxin to induce EAE. A The graph shows the clinical score of EAE (n = 5 per group). B Concentration of IFN-γ, IL-17, GM-CSF, and IL-10 in serum was measured by ELISA on day 20. C The cells infiltrating the central nervous system (CNS) were re-stimulated with MOG(35-55) peptide directly ex vivo, and the intracellular production of IFN-γ and IL-17A by CD4+ T cells was determined. Pooled data are presented in the right panel. (D) Flow cytometry of Treg cell (CD25+ Foxp3+) in CNS of Rnf213fl/fl and Rnf213fl/flCD4Cre mice on days 20 during EAE. Pooled data are presented in the right panel. Data shown are the mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001. P values were calculated using a two-sided, unpaired Student’s t-test (A–D). N = 5 (B–D) repeats from three independent experiments. Source data are provided as a Source Data file.

Back to article page