Fig. 2: RNF213 promoted Treg cell differentiation in a T cell-intrinsic manner in vivo. | Nature Communications

Fig. 2: RNF213 promoted Treg cell differentiation in a T cell-intrinsic manner in vivo.

From: RNF213 promotes Treg cell differentiation by facilitating K63-linked ubiquitination and nuclear translocation of FOXO1

Fig. 2

A Schematic of experimental design of competitive adoptive CD4+ T cell transfer assays. B The cells infiltrating the central nervous system (CNS) were harvested, and the percentages of WT (CD45.1+) and Rnf213−/− (CD45.2+) in the CD4+ T cell populations were determined. C, D Flow cytometry of IFNγ+, IL-17A+ CD4+ cells (C) or CD4+ CD25+ Foxp3+ Treg cells (D) in CNS of WT and Rnf213−/− mice on days 20 during EAE. E QPCR analysis of Foxp3, TGF-β1, and IL-10 in CD4+ cells isolated from CNS of WT and Rnf213−/− mice. Data shown are the mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001. P values were calculated using a two-sided, unpaired Student’s t-test in (C–E). N = 5 (C–E) repeats from three independent experiments. Source data are provided as a Source Data file.

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