Fig. 6: RNF213 promotes FOXO1 K63-linked polyubiquitination and activity through Ubiquitination activity. | Nature Communications

Fig. 6: RNF213 promotes FOXO1 K63-linked polyubiquitination and activity through Ubiquitination activity.

From: RNF213 promotes Treg cell differentiation by facilitating K63-linked ubiquitination and nuclear translocation of FOXO1

Fig. 6

A HEK293T cells were co-transfected with a variety of ubiquitin mutants and other indicated plasmids for ubiquitination analysis. B Immunoprecipitation (IP) and immunoblot (IB) analysis of HEK293T cells that were transfected with indicated plasmids for 24 h. DZR double zinc ribbon, AAA ATPases associated with a variety of cellular activities, RING RING finger ___domain. C Luciferase activity of HEK293T cells transfected with a luciferase reporter driven by the Foxp3 promoter and expressing vector alone or FOXO1, RNF213, and RNF213ΔRING (lacking RING ___domain). D Flow cytometry of Foxp3 in CD4+ T cells infected with control retrovirus (Vector) or retrovirus expressing RNF213 or RNF213ΔRING differentiated under Treg-polarizing conditions. Pooled data are presented in the right panel. Data shown are the mean ± SD. *P < 0.05, **P < 0.01, and ***P < 0.001. P values were calculated using a two-sided, unpaired Student’s t-test in (C, D). N = 3 (C), n = 5 (D) repeats from three independent experiments. Source data are provided as a Source Data file.

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