Fig. 3: Phenotypes of FoxO1-cKO, Tg-Mst1, and Tg-Mst1-FoxO1-cKO bigenic mice.
a Quantification of apoptotic cardiomyocytes by counting TUNEL-positive nuclei. The number of TUNEL-positive myocytes was expressed as a percentage of total nuclei, determined by DAPI staining. The experimental data were normalized by the values obtained from control cardiomyocytes without adenovirus transduction (n = 6). *(1–3) vs. Ad-LacZ_H2O2(−), †(1–2) vs. Ad-LacZ_H2O2(+), #(1–2) vs. Ad-Mst1. b Representative pictures of M-mode echocardiographic imaging in FoxO1-cKO, Tg-Mst1, Tg-Mst1 crossed with FoxO1-cKO (Tg-Mst1-FoxO1-cKO), and WT mice at 70–80 days old. c Quantitative analyses of left ventricular ejection fraction in FoxO1-cKO (n = 7), Tg-Mst1 (n = 7), Tg-Mst1-FoxO1-cKO (n = 5), and WT (n = 8) mice. d −dP/dt was measured in FoxO1-cKO, Tg-Mst1, Tg-Mst1-FoxO1-cKO, and WT mice (n = 4). e Lung weight/tibia length (TL) was measured in FoxO1-cKO, Tg-Mst1, Tg-Mst1-FoxO1-cKO, and WT mice (n = 5). f LV myocyte cross-sectional area was measured. Upper: LV tissue sections were stained with wheat germ agglutinin—Texas red. Lower: the results of quantitative analyses (n = 9). g Fibrosis area in LV tissues was measured. Upper: LV tissue sections were stained by Masson’s trichrome staining. Lower: the results of quantitative analyses (n = 9). h TUNEL-positive myocytes were counted among more than 5000 nuclei in the LV tissues of each animal. Upper: representative pictures of TUNEL and DAPI staining in the LV tissues. Lower: the results of quantitative analyses (n = 15). All experiments were repeated at least three times, with n representing biologically independent replicates. P values were determined by one-way ANOVA followed by Tukey’s multiple comparison test in (a, c–h). Data are mean ± SEM. Source data are provided as a Source Data file.
