Fig. 7: Elimination of senescent CAFs in transplanted PDACs using ABT-199 increases activated CD8+ T cell rates. | Nature Communications

Fig. 7: Elimination of senescent CAFs in transplanted PDACs using ABT-199 increases activated CD8+ T cell rates.

From: Senescent cancer-associated fibroblasts in pancreatic adenocarcinoma restrict CD8+ T cell activation and limit responsiveness to immunotherapy in mice

Fig. 7

a Diagram of experimental design. 6422c1 KPC cells were injected into wt mouse pancreata. Mice were treated with ABT-199 three times a week during tumor growth. b Sections of tumors formed by KPC cells in mice either untreated or treated with ABT-199, co-stained for p16 and for YFP (top), marking epithelial cells, or Pdpn (bottom) marking CAFs. c Relative stromal p16+ area in tumors with or without ABT-199 treatment. Values were measured by image analysis and normalized to mean of controls. n = 7 UNT, n = 6 ABT-199, >1 mm2/tumor. d Percentage of p16+ cells out of Pdpn+ CAFs in same samples, scored by image analysis. e Tumor weights upon sacrifice, in mice treated with ABT-199 or untreated. f Quantification of indicated immune cell populations in tumors formed in untreated and in ABT-199-treated mice, scored by FACS. g Percentages of activated CD8+ T cells in same mice, scored with indicated markers by FACS. h Quantification of indicated additional immune cell populations in tumors formed in untreated and in ABT-199-treated mice, scored by FACS. n = 7,6 tumors in (c–h). All values indicate mean ± SEM. t test. Scale bars = 20 μm.

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