Fig. 6: DmxA is essential for the symmetric allocation of polarity proteins to the daughters during cytokinesis.

a RomR-mCherry localization during the cell cycle. Left panels, epifluorescence and phase-contrast images from representative time-lapse microscopy of WT and ΔdmxA cells. Images were recorded every 10 min; arrowheads indicate completion of cytokinesis. Right panels, histogram quantification of RomR-mCherry localization pattern based on time-lapse microscopy. RomR-mCherry polar fluorescence was quantified 30 min before and after division in the predivisional cell (gray) and daughters (yellow/pink) and ω was calculated as indicated. Daughter cells were sorted based on their ω with the most symmetric in yellow and the other in pink. Localization patterns were binned as unipolar, asymmetric, and symmetric from the ω values as in Fig. 5b. Line and error bars indicate the median±MAD of the indicated number of cells from three biological replicates, number of cell division events per replicate in brackets. Note that in the ΔdmxA mutant, two daughters only had a diffused signal. Schematics show dominant localization patterns of RomR-mCherry in WT and ΔdmxA cells during the cell cycle. b Localization of RomR-mCherry, MglB-mVenus, and MglA-mVenus in WT and ΔdmxA cells. Left panels, representative snapshot images. Analysis of snapshot images of WT (gray) and ΔdmxA (blue) were done as in Fig. 5b. c SgmX-mVenus localization in representative moving WT and ΔdmxA cells. Images were recorded every 30 s. White arrows indicate reversals. Source data are provided as a Source Data file.