Fig. 7: Isoform switching in Bin1 associated with tau pathology in TG mice. | Nature Communications

Fig. 7: Isoform switching in Bin1 associated with tau pathology in TG mice.

From: Long-read transcript sequencing identifies differential isoform expression in the entorhinal cortex in a transgenic model of tau pathology

Fig. 7

A A cluster dendrogram depicting the repertoire of Bin1 isoforms detected using targeted long-read sequencing in the entorhinal cortex from WT and TG mice. Each row corresponds to a distinct isoform and each column represents a known exon. B A bar-plot of the number of isoforms in which a known exon (mm10, GENCODE) is skipped. The numbering of each known exon is determined from flattening the reference transcripts (mm10) using FICLE. C Visualization of the reference and selected novel isoforms of Bin1 characterized by exon skipping events (ES), intron retention (IR), alternative promoter use (AP) and novel cryptic exons (CE). Isoforms are coloured by structural category (pink – NIC, light pink – NNC). D Transcript expression, determined from normalized ONT full-length read counts, of two known isoforms: LR.Bin1.99, (Bin1-201, ENSMUST00000025239.8) and LR.Bin1.224 (Bin1-205, ENSMUST00000234496.1). Isoforms are coloured with reference to C. E Bin1 gene expression (normalized ONT full-length read counts) in WT (gray) and TG (black) mice across age. Isoform usage (proportion) of LR.Bin1.224 stratified by (F) genotype and (G) age (n = 18). For the shown box-plot, the middle box represents the interquartile range (IQR), the middle line represents the median, and the whisker lines represent the minimum (quartile 1 – 1.5 × IQR) and the maximum (quartile 3 + 1.5 × IQR).

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