Fig. 1: asORFs are more likely to exist than igORFs of identical lengths and composition.
From: How antisense transcripts can evolve to encode novel proteins
A A hypothetical antisense codon (bottom sequence, orange) can overlap with sense ORF (top sequence, black) in three different frames. Arrows indicate the direction of translation and vertical bars indicate base complementarity. Adjacent codons in the sense ORF are demarcated with horizontal square brackets. B The probability of asORFs in frame 1 relative to that of igORFs (\({\log }_{2}\) ratio, vertical axis), for different values of GC content of the ORFs (line colours yellow = 30%, blue = 40%, red = 50%, purple = 60%). We do not show asORFs in frames 0 and 2 because their probabilities are identical to that of igORFs. The probability of asORFs relative to that of igORFs (\({\log }_{2}\) ratio, vertical axis), calculated using frequencies of short DNA sequences from C the yeast genome, and D the fruitfly genome. Frames 0, 1 and 2 are denoted by dotted, solid and dashed lines, respectively. Horizontal axes in panels (B)–(D) show the length of the ORFs. We only show asORFs that overlap completely with the sense ORF. Source data are provided as a Source data file.
