Fig. 8: Differential proteomics reveal low abundance of all multiprotein complex TriC/CCT components as a hallmark of pWNT MB. | Nature Communications

Fig. 8: Differential proteomics reveal low abundance of all multiprotein complex TriC/CCT components as a hallmark of pWNT MB.

From: Multiomic profiling of medulloblastoma reveals subtype-specific targetable alterations at the proteome and N-glycan level

Fig. 8

A Differentially abundant proteins when comparing pWNT (n = 19) to pG3myc (n = 26) MB (two-tailed, unpaired t test, p value < 0.05; log2FC > 1.5). B GSEA showing the top 10 up or downregulated pathways comparing pG3myc MB to pWNT (GSEA differential expression analysis normalized enrichment score (NES), p < 0.05, FDR < 0.25). C Mean protein abundancies, gene expression values and methylation at CpG sites for all components of the tailless complex polypeptide 1 ring complex/Chaperonin containing tailless complex polypeptide 1 (TriC/CCT) per proteome subtype in matched cases (npWNT = 4, npSHHt = 14, npSHHs = 4, npG3 = 6, npG4 = 17, np3Myc = 11, data are presented as mean values ± SD. Left: Heatmaps. Middle: Quantification (two-tailed, unpaired t test). Right: p values when comparing subtypes (ppWNTvspSHHt < 0.0001, ppWNTvspSHHs < 0.0001, ppWNTvspG3 < 0.0001, ppWNTvspG3myc < 0.0001, ppWNTvspG4 < 0.0001, ppSHHtvspSHHs < 0.001, ppSHHttvspG3 < 0.0001, ppSHHtvspG3myc < 0.0001, ppSHHtvspG4 < 0.01, ppSHHsvspG3 < 0.01, ppSHHsvspG3myc < 0.0001, ppSHHsvspG4 < 0.05, ppG3vsG4 = n.s., ppG3vspG3myc < 0.0001, ppG4vspG3myc < 0.0001). D Correlation plot displaying mean correlation for each component in all three omic types. E Circus plot displaying correlations ≥0.7 for each component’s protein, gene and CpG site. Only CCT2 significantly correlated on all three levels. n represents biologically independent human samples. NS = not significant.

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