Fig. 3: Degradation characteristics of LL-K12-18.
From: Dual-site molecular glues for enhancing protein-protein interactions of the CDK12-DDB1 complex
Protein levels of CDK12 and cyclin K after 4 h treatment with LL-K12-18 (a) and SR-4835 (b) in MDA-MB-231 cells. c DC50 curves quantified by western blot assay (n = 3, Supplementary Fig. 11). Data are presented as mean values +/- SD. Time course of cyclin K degradation by 50 nM LL-K12-18 (d)and SR-4835 (e). Cyclin K levels rescued by 1 h pre-treatment with 10 μM MLN-4924 (f), 1 μM MG132 or reduced levels of DDB1 knockdown by shRNA(g). All blots in one subfigure came from an identical sample and were processed in parallel. Similar results were found in two repeated assays.
