Fig. 7: An exemplar teaching module using the frugal CRISPRkit.
From: A frugal CRISPR kit for equitable and accessible education in gene editing and synthetic biology
a Smartphone images of combinations of different chromoproteins in CFS reactions transferred to a parafilm. Conditions are listed below each droplet. b Schematic of a ‘guess-and-test’ experiment. The question asked is which genes are in the tube? A tube containing unknown plasmids encoding one or more chromoproteins and the frugal CRISPR kit are provided to the students. c The raw parafilm and tube images from one ‘guess-and-test’ experiment. d Experimental data from the ‘guess-and-test’ CRISPRkit experiment analyzed by CRISPectra. Three biological replicates calculated from mean of two technical replicates are plotted for each group. The bars represent the mean of biological replicates, and the error bars represent the standard deviation. Two one-way ANOVA were performed; p values generated by comparison of chosen conditions to no-guide control via Dunnett’s multiple comparisons test are indicated above the chosen condition, above the fold of repression. e Experimental data from using the frugal CRISPRkit experiment on sfGFP. Three biological replicates calculated from the mean of two technical replicates are plotted for each group. The bars represent the mean of biological replicates, and the error bars represent the standard deviation. Two-tailed Student’s t-test was performed; p values are indicated above the no guide and guide condition bars. Source data are provided as a Source Data file.
