Fig. 5: NbERD15La negatively regulated RxLR23KM-triggered plant immunity.
pBinGFP2:RxLR23KM, pBinGFP2:NbERD15La, NbERD15La + RxLR23KM, and pBinGFP2 were transiently expressed in S (TRV:NbERD15La) and NS (TRV:GFP) N. benthamiana leaves alone. The leaves were inoculated with P. capsici zoospores after expression of all these constructs at 24 h. A Typical lesion images of leaves were photographed under UV irradiation at 48 hpi (n = 30 samples). B H2O2 accumulation in N. benthamiana leaves was measured by DAB staining at 36 h (n = 30 samples). C Mean diameter of lesions was measured at 48 hpi. Values are presented as mean ± SD, n = 30 samples. D Relative biomass of P. capsici was measured by qPCR at 48 hpi. Pcβ-actin and NbEF1α were identified as the most suitable reference genes for normalization. The ratio in the leaves inoculated with GFP was assigned to value of 1.0. Values are presented as mean ± SD, n  = 3 independent experiments. E Relative levels of DAB staining were examined at 36 h. The relative staining of GFP was assigned to value 1.0. Values are presented as mean ± SD, n = 3 independent experiments. F Expected proteins size was detected by western blots using GFP-antibodies alone. Protein loading is indicated by Ponceau staining (Ponceau S). G Expression levels of both PR1/2 were detected by qRT-PCR at 48 hpi. NbEF1α of N. benthamiana was used as constitutively expressed endogenous control. Values are presented as mean ± SD, n = 3 independent experiments. H, I The variations of SA or ABA content were examined by HPLC-MS in N. benthamiana leaves after expressing each of these constructs at 24 h, respectively. Values are presented as mean ± SD, n = 3 independent experiments. The data in (C–E, G–I) were analyzed by ANOVA one-way comparison followed by least significant difference (LSD) test and different letters above the bars indicate a significant difference at p < 0.05. These experiments (A–C, E, F) were repeated three times with similar results. Source data are provided as a Source Data file.
