Fig. 6: MICALs share similar autoinhibition and relief mechanisms with MICAL1.
From: Autoinhibition and relief mechanisms for MICAL monooxygenases in F-actin disassembly
a Sequence alignments of MICAL proteins across different species. Several highly conserved residues at the interdomain interfaces, critical for autoinhibition formation in MICAL1, are indicated. The complete sequence alignments are included in Supplementary Fig. 8. b Confocal imaging of HeLa cells overexpressing GFP-tagged MICAL3 and its variants. F-actin was stained by fluorescence-labeled phalloidin. c, d Quantitative analyses of cellular F-actin levels (c) and cell size (d) in HeLa cells overexpressing GFP-tagged MICAL3 and its variants. Data from 20 cells were analyzed for each condition. e Proposed model illustrating the transition from the autoinhibited/closed to open states of MICALs by dual Rab-binding. The conformational changes containing moving away of the CH and LIM from RBD induced by the H3 helical structure formation upon the first Rab binding and the rotation of H1 upon the second Rab binding, away from the MO, are indicated by red arrows.
