Fig. 6: Function of the SRiApt-1 aptamer on cell phenotypes. | Nature Communications

Fig. 6: Function of the SRiApt-1 aptamer on cell phenotypes.

From: Blocker-SELEX: a structure-guided strategy for developing inhibitory aptamers disrupting undruggable transcription factor interactions

Fig. 6

a GSEA for KEGG gene sets associated with the apoptosis pathway (NES = 0.83, p < 0.05) after treated with SRiApt-1 aptamers. Data were analyzed by one-tailed Fisher’s exact test. b Representative apoptotic curves of HCT116 cells treated with SRiApt-1 or RC control for 3 days under Annexin V staining. (n = 3, mean ± SD. Two-sided Student’s t-test, 95% confidence interval = 41223-55705) Source data are provided as a Source Data file. c Cell viability assessed using the CCK8 assay when cells were treated with SRiApt-1-m or RC aptamers at a working concentration of 1 µM. The result is determined from three biological replicates, each containing three technical replicates (biological replicates n = 3, mean ± SD, Two-sided Student’s t-test). The 95% confidence interval is −78.25 to −19.65. Source data are provided as a Source Data file.

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