Fig. 5: Flg22 stimulates removal of proline from the Arabidopsis leaf apoplast. | Nature Communications

Fig. 5: Flg22 stimulates removal of proline from the Arabidopsis leaf apoplast.

From: Transporter-mediated depletion of extracellular proline directly contributes to plant pattern-triggered immunity against a bacterial pathogen

Fig. 5

a Arabidopsis Col-0 leaves were infiltrated with 500 µM 13C-proline and 164 µM ribitol. Apoplastic wash fluid (AWF) was isolated from treated leaves at time points indicated, and the abundance of 13C-proline and ribitol in AWF measured by GC-MS. Graphed are means ± SE of 13C-proline or ribitol peak area normalized to levels of myristic acid added as an external standard, n = 3. b, c Col-0 (b) and sid2/pad4/ein2/pad4 (QKO) (c) plants were treated with 100 nM flg22 or a mock treatment for six hours. The plants were then infiltrated with 500 µM 13C-proline and 164 µM ribitol. AWF was recovered from treated leaves at time points indicated. Graphed are means ± SE of 13C-proline peak area normalized to ribitol peak area, n = 3. Asterisks in all panels denote significance based on two-sided t-test vs t = 0 measurements, *p < 0.05, ***p < 0.001, ns is p > 0.05. Data in each panel were pooled from three independent experiments.

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