Fig. 3: The characterization of MNB-Pyra Nbs and its spectral analysis. | Nature Communications

Fig. 3: The characterization of MNB-Pyra Nbs and its spectral analysis.

From: Self-reporting photodynamic nanobody conjugate for precise and sustainable large-volume tumor treatment

Fig. 3

a Schematic representation of the preparation of MNB-Pyra Nbs. b SDS-PAGE analysis of the protein conjugate MNB-Pyra Nbs. c Protein mass spectrometry analysis of MNB-Pyra Nbs. d Hydrodynamic size of 7D12-fGly and MNB-Pyra Nbs by dynamic light scattering. e, f Normalized absorbance and fluorescence spectra of MNB-Pyra (10 µM) and MNB-Pyra Nbs (5 µM). g Fluorescence recovery of MNB-Pyra Nbs after 630 nm light irradiation (30 mW/cm2), n  = 3 experimental replicates, data are shown as mean ± SD. Statistical significance in (g) was calculated via one tailed Student’s t test. P  <  0.05 is considered to be statistically significant. Source data are provided as a Source Data file.

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