Table 1 Thermodynamic parameters and dissociation constants (Kd) for DNA binding of Meis1-HD at 298 K.a

From: Molecular basis of facilitated target search and sequence discrimination of TALE homeodomain transcription factor Meis1

meisDNA

Specific binding

Non-specific binding

 

ΔHSB (kcal/mol)

−TΔSSB (kcal/mol)

n

Kd,SB (nM)

ΔHNB (kcal/mol)

−TΔSNB (kcal/mol)

n

Kd,NB (μM)

WT

−10.18 ± 0.08

−0.49 ± 0.03

0.97 ± 0.01

15.2 ± 2.8

−5.60 ± 0.63

−2.04 ± 0.77

1.07 ± 0.03

2.55 ± 0.64

T1C

−7.00 ± 0.20

−3.11 ± 0.34

0.99 ± 0.03

39.8 ± 9.4

−5.56 ± 0.38

−2.16 ± 0.50

1.01 ± 0.07

2.22 ± 0.49

G2A

−8.60 ± 0.40

−0.89 ± 0.32

1.02 ± 0.03

112.9 ± 16.4

−5.06 ± 0.36

−2.36 ± 0.59

0.99 ± 0.03

3.75 ± 1.41

A3G

−5.89 ± 0.17

−3.81 ± 0.15

0.97 ± 0.03

78.1 ± 2.6

−5.29 ± 0.49

−2.32 ± 0.66

0.99 ± 0.01

2.68 ± 0.75

C4T

−8.35 ± 0.46

−1.27 ± 0.43

0.98 ± 0.01

90.2 ± 4.7

−5.40 ± 0.09

−2.38 ± 0.11

1.03 ± 0.05

1.96 ± 0.08

A5G

−7.46 ± 0.09

−2.51 ± 0.05

0.98 ± 0.01

49.7 ± 4.4

−4.97 ± 0.07

−2.76 ± 0.42

1.02 ± 0.07

2.36 ± 1.31

  1. aSample conditions: 10 mM sodium phosphate (pH 6.0), 100 mM NaCl.
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