Fig. 5: Verification of EMG-based muscle activity with NMJ analysis in ALS model rats. | Nature Communications

Fig. 5: Verification of EMG-based muscle activity with NMJ analysis in ALS model rats.

From: All-in-one wearable drug efficacy assessment systems for bulbar muscle function using amyotrophic lateral sclerosis animal models

Fig. 5

A Representative images of innervated, partially innervated, or denervated NMJs. Pre-synaptic terminals (red fluorescent image) were stained with anti-neurofilament and anti-synaptic vesicle antibodies, and post-synaptic terminals were probed with FITC-conjugated α-bungarotoxin. Scale bar: 10 µm. More than 100 NMJs were analyzed from each muscle of each animal. B The innervation status of NMJs ( >100 NMJs) in digastric muscles at 28 weeks old of each group was analyzed. N = 3 for WT and n = 4 for ALS or ALSD group. Data were analyzed using two-way ANOVA with Tukey’s multiple comparison test. ** indicates p = 0.002 (WT vs ALS in innervation), * indicates p = 0.0351 (WT vs ALS in denervation), and #indicates p = 0.0251 (ALS vs ALSD in innervation). Error bars indicate the standard error of the mean. C Correlation between denervation rates at 28 weeks old and muscle activity at 27 weeks old of digastric muscles. Each dot indicates an individual rat in the group.

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