Fig. 6: A neural rosette formation assay reveals abnormal mitotic spindle orientations upon loss of DENND5A. | Nature Communications

Fig. 6: A neural rosette formation assay reveals abnormal mitotic spindle orientations upon loss of DENND5A.

From: Loss of symmetric cell division of apical neural progenitors drives DENND5A-related developmental and epileptic encephalopathy

Fig. 6

a Sample images showing the orientation of apical progenitor cell division in WT and DENND5A KO rosettes. Green = Ki67, red = γ-tubulin, cyan = F-actin, blue =  DAPI. Scale bars = 50 µm, inset = 10 µm. Dotted lines outline the F-actin-positive lumen. b Quantification of mitotic spindle angles measured from n = 85 WT and n = 81 KO dividing cells from two independent experiments, analyzed via two-tailed Mann-Whitney U test (Z = −7.122, p = 1.07 × 10−12). c Pie charts showing the proportion of dividing cells with mitotic spindle angles falling within various ranges. d Overexpression of DENND5A in NPCs. Green = GFP-DENND5A, cyan = TGN46, red = γ-tubulin. Scale bars = 10 µm. Results were reproduced in two independent experiments. Source data for (b, c) are provided as a Source Data file.

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