Fig. 4: Mo/MΦ display a pro-healing transcriptomic profile upon Treg delivery.
From: Local administration of regulatory T cells promotes tissue healing
a Wildtype C57BL6/J mice with bone, muscle or skin injuries were treated with fibrin hydrogel only or hydrogel containing exogenous Tregs. b–f Endogenous Mo/MΦ from injured tissues were sorted for RNA sequencing on D4 and D7 post-injury. Heat maps depicting standardised gene expression values of selected significantly upregulated and downregulated genes (FDR adjusted p value < 0.05) in Mo/MΦ from Treg-treated bone (b), muscle (c), and skin (d) injuries, compared to untreated controls (n = 3 mice/tissue per time point; individual replicates are shown). Colour key denotes the functional category of the genes. Gene ontology terms depicting enriched biological processes in significantly upregulated (e) and downregulated (f) genes in Treg-treated tissues, from both D4 and D7 macrophages combined (FDR < 0.01, adjusted by Benjamini–Hochberg correction). (Mo/MΦ: monocytes/ macrophages). a Created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license (https://creativecommons.org/licenses/by-nc-nd/4.0/deed.en).
