Fig. 7: Tumor-tumor network formation and glioma cell proliferation in cortex and corpus callosum.
From: Deep intravital brain tumor imaging enabled by tailored three-photon microscopy and analysis
a Brain tumor networks in the CC (left) and in the cortex (right) shown as 3D renderings in green. CC and cortex imaging were performed with 3PM and 2PM, respectively. Each network is visualized as network orientation in the bottom right corner. The network is colored based on the local orientation. CC imaging depth: z = 840-950 µm. b Rose plot of overall orientation of the tumor cell network in the CC (n = 271985 local network orientation values from n = 6 brain tumor networks from n = 5 mice). c The standard deviation of tumor network orientation is compared in the CC to the cortex. (n = 692 slices from n = 6 brain tumor networks from n = 5 mice, Mann-Whitney test, shown as median +/- quartile, whiskers: min/max within 1.5 IQR). d Top: MIP time-lapse imaging of GBMC division in the CC. White arrowhead: GBMC before cell division. Yellow and purple arrowhead: Daughter GBMCs after cell division. The asterisk points at a newly grown TM after cell division. Post-processed with denoising and “clear outside” function in ImageJ/Fiji. Bottom: 3D rendering of another cell in the CC before and after cell division. The arrowheads point to the somata of the cell before division and the two daughter cells after division, the arrows point to the TMs (n = 18 cell divisions from n = 7 experiments in 6 mice in 2 PDX models (S24 and T269)). Gamma values were adjusted for 3D-visualization in (a, d). Source data are provided as a Source Data file.
