Fig. 2: Intratumor microbiota induced PDA TME immunosuppression and restrained α-PD-L1 immunotherapy efficacy. | Nature Communications

Fig. 2: Intratumor microbiota induced PDA TME immunosuppression and restrained α-PD-L1 immunotherapy efficacy.

From: Probiotics functionalized with a gallium-polyphenol network modulate the intratumor microbiota and promote anti-tumor immune responses in pancreatic cancer

Fig. 2

a Subcutaneous (S.c.) tumor and b orthotopic tumor growth curves after α-PD-L1 treatment (n = 9 mice). c Concentration of LPS in S.c. and orthotopic PDA tumors (n = 4 samples). Content of d Proteobacteria, Firmicutes bacteria, and e LPS in pancreatic tumors and healthy pancreatic tissues (n = 3 samples). f Bioluminescence images of orthotopic PDAC mice treated with α-PD-L1 or α-PD-L1 plus polymyxin B (PmB) (n = 6 mice). g Immuno-fluorescence staining of intratumor T cell infiltration treated with α-PD-L1 or α-PD-L1 plus PmB. The scale bar is 50 μm. Experiments were performed three times with similar results (n = 6 mice). h Concentration of LPS in tumors treated with α-PD-L1 or α-PD-L1 plus PmB (n = 4 samples). i Infiltration of intratumor immune cells in PDA tumors with and without LPS stimulation (n = 3 samples). Significance between two groups was calculated using two-tailed Student’s t-tests (a–e, h, and i). Data are means ± sem. n.s. means no significance. Source data are provided as a Source Data file.

Back to article page