Fig. 1: Sequence analysis reveals two distinct groups of Class III lanthipeptide modification enzymes.

a Maximum likelihood phylogeny of Class III lanthipeptide modification enzymes identifies two prominent subclasses, referred to as Class III-a (purple) and Class III-b (green). b Sequence alignment of the cyclase ___domain from Class III-a enzyme ThurKC and Class III-b enzyme LanKCs. The residues found to be crucial for cyclase ___domain activity in Class III-a ThurKC are largely absent in Class III-b LanKCs.