Fig. 7: Transcriptome analysis identified NOSR-1 targets.

A Schematic of the animal with or without nucleolar stress. B Venn diagrams reveal the overlapping target genes that are upregulated or downregulated under three nucleolar stress conditions. C–E Heatmap of the standardized fragments per kilobase of transcript per million mapped reads (FPKM) for the 11 coupregulated target genes in the indicated animals. The expression levels are indicated by the color bar. F DIC and fluorescence microscopy images of hypodermal nuclei in exos-10(ust242);mCherry::DIS-3 worms after knockdown of the indicated genes by RNAi (scale bar, 5 μm). The yellow dashed line represents the nucleus, and the yellow arrows indicate NoSB. Three times were repeated independently with similar results. Images were taken by the Leica THUNDER imaging System.