Fig. 2: Endogenous genes activated by dCas9-VP64-IDR.
From: Specific multivalent molecules boost CRISPR-mediated transcriptional activation
a–d Relative mRNA expression of NTF3, ASCL1, MYOD1, and IL1RN in HEK293T cells transfected with dCas9-VP64 or dCas9-VP64-FUS and a single guide RNA (gRNA) targeting each gene promoter as well as a scrambled gRNA (gScr) control. Data are shown as mean ± SD (n = 3 independent experiments). Statistical significance was determined by two-sided Welch’s t-test. e, f Relative mRNA expression of NTF3 and ASCL1 in HEK293T cells transfected with dCas9-VPR or dCas9-VPR-FUS and a gRNA targeting each gene promoter. Data are shown as mean ± SD (n = 3 independent experiments). Statistical significance was determined by two-sided Welch’s t-test. g, h Relative mRNA expression of NTF3 and ASCL1 in HEK293T cells transfected with dCpf1-VP64 or dCpf1-VP64-FUS and a gRNA targeting each gene promoter. Data are shown as mean ± SD (n = 3 independent experiments). Statistical significance was determined by two-sided Welch’s t-test. Source data are provided as a Source data file.
