Fig. 6: Transcriptional activation enhanced via enhancing chromatin interactions.
From: Specific multivalent molecules boost CRISPR-mediated transcriptional activation
a Schematic of ABI-mCherry-dCas9-VP64-FUS and PYL-BFP-dCpf1-VP64-FUS fusion proteins targeting an enhancer and a promoter, respectively. In the presence of abscisic acid (ABA), ABI1 and PYL1 dimerize, inducing an artificial chromatin loop between the targeted enhancer and promoter regions. b, c Relative mRNA expression of HBD and HBG1/2 in HEK293T cells expressing ABI1-mCherry-dCas9 fusion proteins targeted to the HS2 enhancer along with PYL1-BFP-dCpf1 fusion proteins targeted to the corresponding gene promoters. Cells were treated with and without abscisic acid (ABA). The effector domains of the enhancer- and promoter-targeted fusion proteins are indicated. Data are shown as mean ± SD (n = 3 independent experiments). Statistical significance was determined by two-sided Welch’s t-test. Source data are provided as a Source data file.
