Fig. 6: Role of key residues in the PSFL77-recognition within the 1α3β ___domain of P2X3-ICD. | Nature Communications

Fig. 6: Role of key residues in the PSFL77-recognition within the 1α3β ___domain of P2X3-ICD.

From: Finely ordered intracellular ___domain harbors an allosteric site to modulate physiopathological function of P2X3 receptors

Fig. 6

A Possible acting mode of PSFL77 within the1α3β ___domain of P2X3-ICD. B, C Representative current traces (B) and pooled data (C) showing the effect of PSFL77 (100 μM) on WT rP2X3 and its mutants. The y axis denotes the fold increase in current induced by PSFL77, with each open circle on the scatter plot representing an individual measurement (mean ± S.E.M., n = 3 (rP2X3E363A), 5 (rP2X3V361A, rP2X3R356A and rP2X3Y353W), 7 (rP2X3V16I, rP2X3V18A and rP2X3H352A) or 8 (rP2X3WT)). *P < 0.05 and **P < 0.01 vs. rP2X3WT, two-side unpaired t test, P = 0.0114 (rP2X3V16I), 0.0014 (rP2X3V18A), 0.0006 (rP2X3R356A), and 0.0013 (rP2X3Y353W). D Concentration-response curves of PSFL77 on the current amplitude of WT rP2X3 and its mutants induced by 10 μM ATP. The y axis signifies the normalized fold of current increase, expressed as the ratio of the current in the presence of PSFL77 to that in the absence of PSFL77. Data are expressed as mean ± S.E.M. n = 5 (0.3 and 100 μM), 6 (3 and 30 μM) or 7 (1 and 10 μM) independent cells for rP2X3V16I. n = 3 (1, 3, 10, 30, 100 and 300 μM) independent cells for rP2X3V18A. n = 5 (1, 3, 10 and 100 μM), or 4 (30 μM) independent cells for rP2X3V19A. n = 4 (1, 10, 30 and 100 μM), or 3 (3 μM) independent cells for rP2X3V361A. n = 4 (1, 3 and 30 μM), or 6 (10 μM and 100 μM) independent cells for rP2X3Y353W. E Concentration-response curves of PSFL77 to show the impact of PSFL77 on the delay of desensitization in P2X3 induced by 10 μM ATP. The y axis signifies the normalized ratio, derived by dividing the current at 1 s after reaching the maximum current by the maximum current. Curve fitting was performed using solid lines in accordance with the Hill 1 equation (mean ± S.E.M., n = 5 (0.3 μM), 6 (3 and 30 μM) or 7 (1, 10 and 100 μM) independent cells for rP2X3V16I. n = 3 (1, 3, 10, 30, 100 and 300 μM) independent cells for rP2X3V18A. n = 5 (1, 3, 10 and 100 μM), or 4 (30 μM) independent cells for rP2X3V19A. n = 4 (1, 10, 30 and 100 μM), or 3 (3 μM) independent cells for rP2X3V361A. n = 3 (1 μM), 4 (3 and 30 μM), or 5 (10 μM and 100 μM) independent cells for rP2X3Y353W). F Effects of PSFL77 on the concentration-response curve of ATP in P2X3 receptors. Solid lines were fitted to the Hill 1 equation (mean ± S.E.M., n = 6 (3 and 10 μM) or 7 (0.03, 0.1, 0.3 and 1 μM) independent cells for ATP only, n = 3 (1 μM), 5 (0.01, 0.03, 0.1 and 0.3 μM) or 6 (0.003 μM) independent cells for ATP + PSFL77).

Back to article page