Fig. 4: Superposition of nsP3 HSs on tomographic reconstructions of membrane-associated CHIKV RCs. | Nature Communications

Fig. 4: Superposition of nsP3 HSs on tomographic reconstructions of membrane-associated CHIKV RCs.

From: Alphavirus nsP3 organizes into tubular scaffolds essential for infection and the cytoplasmic granule architecture

Fig. 4

a Superposition of nsP1 capping pores and the nsP3 scaffolds on the tomographic reconstruction of CHIKV RCs. Top and lateral views are shown in the top and bottom panels respectively. The volume is shown as a transparent gray surface. nsP1 and nsP3 AUDs are colored by protomer and shown in spheres, the MDs of nsP3 are shown in cartoon and colored in cyan. Their position with respect to the AUDs was determined based on their fitting on the map shown in Fig. 1b (EMD: 17730). The tomographic volume was calculated applying a 12-fold symmetry and consequently has 12 outer volumes on the region which are shown numbered8. The superposition shows the consistent matching in size and shape of AUD helices with the rings above the nsP1 capping pores and the outer spherical volumes have a partial matching with the MDs positions found in HSs. However, the structure of the nsP3 helical scaffold have 11,37 protomers per turn instead of 12, slightly breaking the symmetry with the nsP1 pores and the volume reconstructions. Superposition allows to confidently assign the central ring above nsP1 to the AUDs and the peripheral volumes to the MDs. b same representation of the superposition of capping pores and nsP3 scaffolds on the volume of RCs reported by Tan et al.9. The volume reaches higher resolution and only shows electron density for the AUD region of the nsP3 scaffold. The volume was also calculated imposing C-12 symmetry.

Back to article page