Fig. 7: Nanoparticle delivery of a miR-29a mimetic attenuates T_H1-mediated TNBS Colitis.

T_H1 colitis was induced in WT mice by TNBS dosing as per Materials & Methods. At 24 h and 72 h post-challenge mice were injected IV with respective mimic microRNA in lipid nanoparticle emulsion (5 µg/mouse). a Schematic of experimental design and treatments in TNBS-colitis model. b Histological indices of colitis (inflammatory, injury, and total) were assessed 5 days post-TNBS challenge, pooled 2 experiments, (n = 6–7, two-tailed T test). c Representative H&E micrographs of colons. Scale bar: 100 mm. d At the time of necropsy, purified lamina propria lymphocytes were assessed by Q-PCR for. the expression of T-bet mRNA, normalized to 18S rRNA, (n = 3–4, one-way ANOVA). e Expression of IFNγ mRNA in purified lamina propria T lymphocytes. Data expressed as Mean ±S.E.M from 2 pooled experiments, (n = 3–4, one-way ANOVA). TNF^∆ARE/+ mice manifest with spontaneous ileitis. f Schematic of miR-29a mimetic treatment schedule of TNF^∆ARE/+ mice. g Flow cytometry staining of IFNγ-positive CD4⁺ T cells and number of CD4⁺ T cells as a percentage of total MLN cells at the conclusion of experiment, 2 pooled experiments, (n = 9–10, one-way ANOVA). h Overall histological inflammatory score, 2 pooled experiments, (n = 13, one-way ANOVA). i Representative H&E micrographs of the ileum in control (untreated), mock-treated and miR-29a mimetic treated mice. Scale bar: 100 mm. Male and female mice used in all experiments. Data expressed as Mean ±S.E.M, *p < 0.05, **p < 0.01 vs. indicated. Schematics in (a, f) were created with BioRender.com.