Fig. 3: Overaccumulation of HB21 leads to pollen rupture and male sterility in efd.
From: An epigenetically mediated double negative cascade from EFD to HB21 regulates anther development
a examination of the HB21 expression level in both wild-type and efd buds by RT-qPCR. Data are presented as mean values ±SD. The error bars represent the SDs from three independent biological repeats. Two-tailed Student’s t test was used for statistical analysis of the difference between the wild type and mutants, **p < 0.01 (the p-value is shown in the figure). b in situ hybridisation of HB21 transcripts in the wild type and efd during anther development. T, tapetum; M, pollen mother cell; S5–S11, Stage 5–11; eS6, early Stage 6; lS6, late Stage 6. Bars = 50 μm. c fertility observation of wild-type, efd, hb21 and efd hb21 plants. efd was sterile, while hb21 was fertile. The efd hb21 double homozygous line showed obvious restored fertility compared with that of the efd single mutant. Bars = 1 mm. d RT-qPCR identification of the pollen wall developmental related genes in efd hb21. These genes were downregulated in the efd buds. Data are presented as mean values ±SD. The error bars represent the SDs from three independent biological repeats. Student’s t test was used for statistical analysis of the difference between the wild type and mutants, ***p < 0.001 (all the p-values are shown in the figure).
