Fig. 2: Study flowchart imaging approach and quantification of scan results.

A Consort flowchart diagram of the study. B Schematic and photographic representation of MSOT imaging approach. The imaging probe emitting pulsed laser light was held onto the distal third of the upper arm, scanning the biceps muscle. C Localization of appropriate scan was performed on ultrasound B-mode images. These were used to post-process optoacoustic spectral information. MSOT multispectral optoacoustic tomography, MRI magnetic resonance imaging, ROI region of interest, R-Pact Rasch-built Pompe-specific activity score. This figure was created with BioRender.com released under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International license.