Fig. 5: Multicenter patient cohort comparison proves the applicability and validity of MSOT approach. | Nature Communications

Fig. 5: Multicenter patient cohort comparison proves the applicability and validity of MSOT approach.

From: Non-invasive optoacoustic imaging of glycogen-storage and muscle degeneration in late-onset Pompe disease

Fig. 5

A Interrater analysis comparison of MSOT mean signals for two independent investigators (investigator 1, investigator 2) of two clinical centers (LOPD center 1, LOPD center 2) independently analyzed the same MSOT biceps muscle scans (10 datasets from n = 6 independent biceps muscle regions (n = 3 LOPD) in n = 3 biologically independent subjects). Data collected by center 2. B Interrater analysis comparison of MSOT top 10% signals for two independent investigators (investigator 1, investigator 2) of two clinical centers (LOPD center 1, LOPD center 2) independently analyzed the same MSOT biceps muscle scans (10 datasets from n = 6 independent biceps muscle regions (n = 3 LOPD) in n = 3 biologically independent subject). Data collected by center 2. Dual center comparison (center 1: n = 10 vs. center 2: n = 3) of mean (C) and top 10% (D) MSOT signals. Each filled blue circle displays the mean (C) and top 10% (D) of LOPD patients of center 1, each filled yellow circle displays the mean (C) and top 10% (D) of LOPD patients of center 2. Results of center 1 represent 80 datasets from n = 40 independent biceps muscle regions (n = 20 HV/n = 20 LOPD) in n = 20 biologically independent subjects (n = 10 HV and n = 10 patients with LOPD). Results of center 2 represent 10 datasets from n = 6 independent biceps muscle regions (n = 3 LOPD) in n = 3 biologically independent subjects. Comparison of mean MSOTcol (E), top 10% MSOTcol (F), mean MSOTlip (G), and top 10% MSOTlip (H) between HV and LOPD patients of both centers. Green bar representing HV consists of 40 datasets from n = 20 independent biceps muscle regions of n = 10 biologically independent subjects. Blue bar representing LOPD Center 1 of 40 datasets from n = 20 independent biceps muscle regions of n = 10 biologically independent subjects, yellow bar representing center 2 consists of 10 datasets from n = 6 independent biceps muscle regions (n = 3 LOPD) of n = 3 biologically independent subjects. Ordinary one-way ANOVA was used for statistical analysis. If the assumption of normal distribution was violated, a Kruskal–Wallis test was used. Box plots are defined with a minimum at the 25th percentile, a maximum at the 75th percentile, center at the median value and whiskers at the minimal and maximal data points of each subgroup. MSOT multispectral optoacoustic tomography, HV healthy volunteer, LOPD late-onset Pompe disease patient.

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