Fig. 1: The relay of WNT signaling is mainly mediated by the DEP ___domain of Dishevelled. | Nature Communications

Fig. 1: The relay of WNT signaling is mainly mediated by the DEP ___domain of Dishevelled.

From: Structural basis of Frizzled 4 in recognition of Dishevelled 2 unveils mechanism of WNT signaling activation

Fig. 1

a A schematic diagram of the DVL2 constructs used in this study. b, c The TOPFlash assay for Norrin (b) or WNT3a (c) stimulated FZD4 activity. Data are presented as mean values ± SD; n = 3 independent samples; Two-side T-test. See source data for each exact p value. RLU: relative luciferase unit. d A schematic diagram of the BRET assay used for detecting FZD/DVL interaction. e, f BRET assay of FZD4/DVL2 interaction, e for basal activity and f for MBP-Norrin (10 µg/ml) induced activity. Data are presented as mean values ± SD; n = 3 independent samples; n.s. no significant; *p < 0.05; **p < 0.01; ***p < 0.001; Two-side T-test. See source data for each exact p value. Source data are provided as a Source Data file.

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