Fig. 3: Expression of lipid metabolism-associated genes in Tg6-mice with high EPO compared with WT-mice.
From: Erythropoietin regulates energy metabolism through EPO-EpoR-RUNX1 axis
a, b Images show staining for uncoupling protein 1 (UCP1; yellow) and DAPI (blue) in scWAT from WT-mice and Tg6-mice (a) and quantification of fluorescence intensity by ImageJ (b (t, df = 8.656, 3)). c, d Two-sided unpaired t-test. Scale bar: 200 μm. Gene expression of BAT-associated genes Ucp1, peroxisome proliferator-activated receptor-γ coactivator 1α (Pgc1α), cell death-indicating DNA fragmentation factor a-like effector A (Cidea) and Prdm16 in scWAT (c F(3, 9) = 14.44) and eWAT (d F(3, 9) = 0.1504) from WT-mice and Tg6-mice. e–i Gene expression was determined by real-time quantitative PCR (qPCR) for peroxisome proliferator-activated receptor γ (PPARγ), lipoprotein lipase (LPL), acetyl-CoA carboxylases (ACC1, ACC2), Fas, Lipin1, sterol regulatory element binding protein 1 (Srebf1), and stearoyl-CoA desaturase 1 (Scd1) for scWAT (e F(7, 21) = 41.43), eWAT (f F(7, 21) = 52.60), BAT (g F(7, 21) = 32.11), skeletal muscle (h F(7, 21) = 33.81), and liver (i F(7, 21) = 14.66) for WT-mice and Tg6-mice. Bars indicate WT-mice (gray) and Tg6-mice (red) mice. b–i: n = 4 mice/group, the result shown in figure represent one of three independent experiments. Data shown as mean ± SEM. p values are indicated. b: t-test; c–i: two-way ANOVA with Bonferroni’s multiple comparisons test. Source data are provided as a Source Data file.
