Fig. 4: EPO regulation of lipid metabolism gene expression in WT-mice was not evident in ΔEpoRE mice or mice that lack EpoR in WAT.
From: Erythropoietin regulates energy metabolism through EPO-EpoR-RUNX1 axis

a, b Body weight (a F(2.61, 7.82) = 4.15) and fat mass (b F(1.225, 3.675) = 69.91) were determined for WT-mice treated with PBS (light gray) or EPO (dark gray) and ΔEpoRE mice treated with PBS (light purple) or EPO (purple) for 3 weeks beginning at postnatal 21 days. c–e WT-mice were treated with PBS (light gray) or EPO (dark gray) and ΔEpoRE mice were treated with PBS (light purple) or EPO (purple) for 3 weeks beginning at postnatal 21 days and gene expression determined for Pparγ, Lpl, Acc1, Acc2, Fas, Lipin1, Srebf1, and Scd1 by qPCR in the scWAT (c F(2.397, 7.192) = 113.7), skeletal muscle (d F(1.586, 3.173) = 44.39), and liver (e F(1.884, 3.769) = 15.88). f, g Body weight (f F(10, 50) = 0.577) and fat mass (g F(10, 50) = 2.661) were determined for EpoRAdiponectin-KO mice treated with PBS (gray) or EPO (blue). h–j EpoRAdiponectin-KO mice were treated with PBS (gray) or EPO (blue) for 3 weeks beginning at postnatal 21 days and gene expression for Pparγ, Lpl, Acc1, Acc2, Fas, Lipin1, Srebf1, and Scd1 in scWAT (h F(7, 35) = 0.3032), skeletal muscle (i F(7, 35) = 0.4291), and liver (j F(7, 35) = 0.2572) determined by qPCR. Data shown as mean ± SEM. a–c n = 4; d, e n = 3; f–j n = 6, b–e, h–j: the n means mice per group. The result shown in figure represents one of three independent experiments. p values are indicated. One-way or Two-way ANOVA with Bonferroni’s multiple comparisons test. Source data are provided as a Source Data file.