Fig. 2: Antibody sequence repertoire analysis from OMV-immunised Kymouse platform mice. | Nature Communications

Fig. 2: Antibody sequence repertoire analysis from OMV-immunised Kymouse platform mice.

From: Exploiting human immune repertoire transgenic mice for protective monoclonal antibodies against antimicrobial resistant Acinetobacter baumannii

Fig. 2

a A sunspot plot showing antibody sequence clustering, where spot colour indicates the individual mouse immunised with pooled OMVs from which the antibody sequence originated (n = 4). Individual mice (indicated by nominative code identifier in the index) are represented by colour-matched code in the index. b Sunspot plot showing antibody sequence clustering based on an individual germline IGHV and IGHJ combination utilised. Germline IGHV and IGHJ combination utilised are represented by colour-matched code in the index. c Plot of signal from indirect human IgG1 ELISAs against the OMV cocktail (x-axis) versus unfixed pooled bacteria from which the immunising OMVs were derived (y-axis). d Deposition of the C3b protein on OMVs as a surrogate for primary initiation of the complement cascade (x-axis) versus indirect human IgG1 ELISAs against unfixed pooled bacteria from which the immunising OMVs were derived (y-axis).

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