Table 1 In vitro kinetic characterization of AGE and mutant activities

From: De novo engineering of programmable and multi-functional biomolecular condensates for controlled biosynthesis

Enzymes

Km (μM)

kcat (s−1)

Vmax (μM min−1)

kcat/Km (s–1 M–1)

AGE

49.2

2.3

0.4

46,266.9

AGEOMeY

1448.9

124.7

24.4

86,078.9