Fig. 2: Wax status and leaf permeability of ACL1-D, ACL1KO, and ZH11 plants. | Nature Communications

Fig. 2: Wax status and leaf permeability of ACL1-D, ACL1KO, and ZH11 plants.

From: ACL1-ROC4/5 complex reveals a common mechanism in rice response to brown planthopper infestation and drought

Fig. 2

a–c SEM micrographs of the flag leaf surface of ZH11 (a), ACL1-D (b), and ACL1KO (c). d–f SEM of the leaf sheath surface of ZH11 (d), ACL1-D (e), and ACL1KO (f). Bars in (a–f) are 2 µm. CP, cuticular papillae. g Wax content in leaves of the ACL1-D, ACL1KO, and ZH11 plants revealed by GC-MS. Data are means ± SD (n = 3). h–j Transmission electron micrographs of the cuticle on the adaxial leaf surface of ZH11, ACL1-D, and ACL1KO plants. Bars are 0.5 µm. CW, cell wall. CM, cuticle membrane. k Thickness of the leaf cuticle in ZH11, ACL1-D, and ACL1KO plants. Data are means ± SD (n = 5). l Water-loss efficiency of the leaves of ACL1-D, ACL1KO, and ZH11 plants. Data are means ± SD (n = 8). m Chlorophyll leaching rate of the leaves of ACL1-D, ACL1KO, and ZH11 plants. Data are means ± SD (n = 5). The P-values in (g) and (k–m) were determined by a two-tailed unpaired Student’s t-test. Source data are provided as a Source Data file.

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